:orphan: .. only available via index, not via toctree .. title:: Package Recipe 'bioconductor-ddct' .. highlight: bash bioconductor-ddct ================= .. conda:recipe:: bioconductor-ddct :replaces_section_title: :noindex: The ddCt Algorithm for the Analysis of Quantitative Real\-Time PCR \(qRT\-PCR\) :homepage: https://bioconductor.org/packages/3.14/bioc/html/ddCt.html :license: LGPL-3 :recipe: /`bioconductor-ddct `_/`meta.yaml `_ :links: biotools: :biotools:`ddct`, doi: :doi:`10.1038/nmeth.3252` The Delta\-Delta\-Ct \(ddCt\) Algorithm is an approximation method to determine relative gene expression with quantitative real\-time PCR \(qRT\-PCR\) experiments. Compared to other approaches\, it requires no standard curve for each primer\-target pair\, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect\, analyse and visualize qRT\-PCR results\, for example those from TaqMan SDM software\, mainly using the ddCt method. The pipeline can be either invoked by a script in command\-line or through the API consisting of S4\-Classes\, methods and functions. .. conda:package:: bioconductor-ddct |downloads_bioconductor-ddct| |docker_bioconductor-ddct| :versions: .. raw:: html
1.50.0-01.48.0-01.46.0-11.46.0-01.44.0-01.42.0-01.40.0-11.38.0-01.36.0-0 ``1.50.0-0``,  ``1.48.0-0``,  ``1.46.0-1``,  ``1.46.0-0``,  ``1.44.0-0``,  ``1.42.0-0``,  ``1.40.0-1``,  ``1.38.0-0``,  ``1.36.0-0``,  ``1.34.0-0`` .. raw:: html
:depends bioconductor-biobase: ``>=2.54.0,<2.55.0`` :depends bioconductor-biocgenerics: ``>=0.40.0,<0.41.0`` :depends r-base: ``>=4.1,<4.2.0a0`` :depends r-lattice: :depends r-rcolorbrewer: ``>=0.1-3`` :depends r-xtable: :requirements: .. rubric:: Installation With an activated Bioconda channel (see :ref:`set-up-channels`), install with:: conda install bioconductor-ddct and update with:: conda update bioconductor-ddct or use the docker container:: docker pull quay.io/biocontainers/bioconductor-ddct: (see `bioconductor-ddct/tags`_ for valid values for ````) .. |downloads_bioconductor-ddct| image:: https://img.shields.io/conda/dn/bioconda/bioconductor-ddct.svg?style=flat :target: https://anaconda.org/bioconda/bioconductor-ddct :alt: (downloads) .. |docker_bioconductor-ddct| image:: https://quay.io/repository/biocontainers/bioconductor-ddct/status :target: https://quay.io/repository/biocontainers/bioconductor-ddct .. _`bioconductor-ddct/tags`: https://quay.io/repository/biocontainers/bioconductor-ddct?tab=tags .. raw:: html Download stats ----------------- .. raw:: html :file: ../../templates/package_dashboard.html Link to this page ----------------- Render an |install-with-bioconda| badge with the following MarkDown:: [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat)](http://bioconda.github.io/recipes/bioconductor-ddct/README.html) .. |install-with-bioconda| image:: https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat :target: http://bioconda.github.io/recipes/bioconductor-ddct/README.html