:orphan: .. only available via index, not via toctree .. title:: Package Recipe 'scramble' .. highlight: bash scramble ======== .. conda:recipe:: scramble :replaces_section_title: :noindex: Soft Clipped Read Alignment Mapper :homepage: https://github.com/GeneDx/scramble :license: CC :recipe: /`scramble `_/`meta.yaml `_ :links: doi: :doi:`10.1038/s41436-020-0749-x` SCRAMble identifies clusters of soft clipped reads in a BAM file\, builds consensus sequences\, aligns to representative L1Ta\, AluYa5\, and SVA\-E sequences\, and outputs MEI calls .. conda:package:: scramble |downloads_scramble| |docker_scramble| :versions: ``1.0.2-1``,  ``1.0.2-0``,  ``1.0.1-2``,  ``1.0.1-1``,  ``1.0.1-0`` :depends bioconductor-rsamtools: ``>=2.4.0`` :depends htslib: ``>=1.17,<1.21.0a0`` :depends libgcc-ng: ``>=12`` :depends r-base: ``>=4.0.3`` :depends r-optparse: ``>=1.6.6`` :depends r-rblast: ``>=0.99.1`` :depends r-stringr: ``>=1.4.0`` :requirements: .. rubric:: Installation You need a conda-compatible package manager (currently either `micromamba `_, `mamba `_, or `conda `_) and the Bioconda channel already activated (see :ref:`set-up-channels`). While any of above package managers is fine, it is currently recommended to use either micromamba or mamba (see `here `_ for installation instructions). We will show all commands using mamba below, but the arguments are the same for the two others. Given that you already have a conda environment in which you want to have this package, install with:: mamba install scramble and update with:: mamba update scramble To create a new environment, run:: mamba create --name myenvname scramble with ``myenvname`` being a reasonable name for the environment (see e.g. the `mamba docs `_ for details and further options). Alternatively, use the docker container:: docker pull quay.io/biocontainers/scramble: (see `scramble/tags`_ for valid values for ````) .. |downloads_scramble| image:: https://img.shields.io/conda/dn/bioconda/scramble.svg?style=flat :target: https://anaconda.org/bioconda/scramble :alt: (downloads) .. |docker_scramble| image:: https://quay.io/repository/biocontainers/scramble/status :target: https://quay.io/repository/biocontainers/scramble .. _`scramble/tags`: https://quay.io/repository/biocontainers/scramble?tab=tags .. raw:: html Notes ----- SCRAMble runs as a two\-step process. First cluster\_identifier is used to generate soft\-clipped read cluster consensus sequences. Second\, SCRAMble.R analyzes the cluster file for likely MEIs. Custom wrapper script scramble.sh is provided to help setting location of SCRAMble.R script\, and values for \-\-install\-dir and \-\-mei\-refs params. Download stats ----------------- .. raw:: html :file: ../../templates/package_dashboard.html Link to this page ----------------- Render an |install-with-bioconda| badge with the following MarkDown:: [![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat)](http://bioconda.github.io/recipes/scramble/README.html) .. |install-with-bioconda| image:: https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat :target: http://bioconda.github.io/recipes/scramble/README.html