- recipe bioconductor-hsmmsinglecell
Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts (HSMM)
- Homepage:
https://bioconductor.org/packages/3.16/data/experiment/html/HSMMSingleCell.html
- License:
Artistic-2.0
- Recipe:
Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum-induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell.
- package bioconductor-hsmmsinglecell¶
- Versions:
1.18.0-0,1.17.0-0,1.14.0-1,1.14.0-0,1.12.0-0,1.10.0-1,1.10.0-0,1.9.0-0,1.8.0-0,1.18.0-0,1.17.0-0,1.14.0-1,1.14.0-0,1.12.0-0,1.10.0-1,1.10.0-0,1.9.0-0,1.8.0-0,1.6.0-0,1.4.0-1,1.4.0-0,1.2.0-0,0.114.0-0,0.112.0-1,0.112.0-0,0.110.0-1,0.110.0-0- Depends:
bioconductor-data-packages
>=20221112r-base
>=4.2,<4.3.0a0
- Required By:
Installation
With an activated Bioconda channel (see set-up-channels), install with:
conda install bioconductor-hsmmsinglecell
and update with:
conda update bioconductor-hsmmsinglecell
or use the docker container:
docker pull quay.io/biocontainers/bioconductor-hsmmsinglecell:<tag>
(see bioconductor-hsmmsinglecell/tags for valid values for
<tag>)
Download stats¶
Link to this page¶
Render an 
badge with the following MarkDown:
[](http://bioconda.github.io/recipes/bioconductor-hsmmsinglecell/README.html)
