recipe virasign

Virasign is a viral taxonomic classification tool designed for nanopore sequencing data.

Homepage:

https://github.com/DaanJansen94/virasign

Documentation:

https://github.com/DaanJansen94/virasign/blob/main/README.md

License:

GPL / GPL-3.0-or-later

Recipe:

/virasign/meta.yaml

Virasign (Viral Read ASSIGNment) is a viral taxonomic classification and reference selection tool for nanopore data. It maps long-read sequencing data (via minimap2) against viral databases (RVDB, RefSeq, or custom accessions) and performs taxonomic classification to identify viral species. Virasign generates comprehensive interactive HTML reports with filterable tables, charts and heatmaps. For each identified virus, Virasign also provides the closest reference sequence, mapped reads in FASTQ format, and BAM files which can be used to easily generate a consensus genome and visualize data (e.g., IGV). Virasign includes options to blind yourself from certain incidental findings (such as HIV, Hepatitis viruses, HTLV, EBV, CMV, HPV) when wanted, ensuring these findings do not appear in any output files, in line with consent guidelines and ethical research practices.

package virasign

(downloads) docker_virasign

Versions:

0.0.2-0

Depends:
  • on curl

  • on gzip

  • on minimap2 2.24.*

  • on mmseqs2

  • on python >=3.7,<3.14

  • on samtools >=1.17

  • on seqtk 1.3.*

Additional platforms:

Installation

You need a conda-compatible package manager (currently either pixi, conda, or micromamba) and the Bioconda channel already activated (see Usage). Below, we show how to install with either pixi or conda (for micromamba and mamba, commands are essentially the same as with conda).

Pixi

With pixi installed and the Bioconda channel set up (see Usage), to install globally, run:

pixi global install virasign

to add into an existing workspace instead, run:

pixi add virasign

In the latter case, make sure to first add bioconda and conda-forge to the channels considered by the workspace:

pixi workspace channel add conda-forge
pixi workspace channel add bioconda

Conda

With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run:

conda install virasign

Alternatively, to install into a new environment, run:

conda create -n envname virasign

with envname being the name of the desired environment.

Container

Alternatively, every Bioconda package is available as a container image for usage with your preferred container runtime. For e.g. docker, run:

docker pull quay.io/biocontainers/virasign:<tag>

(see virasign/tags for valid values for <tag>).

Integrated deployment

Finally, note that many scientific workflow management systems directly integrate both conda and container based software deployment. Thus, workflow steps can be often directly annotated to use the package, leading to automatic deployment by the respective workflow management system, thereby improving reproducibility and transparency. Check the documentation of your workflow management system to find out about the integration.

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