recipe bioconductor-crisprseek

Design of target-specific guide RNAs in CRISPR-Cas9, genome-editing systems

Homepage

https://bioconductor.org/packages/3.13/bioc/html/CRISPRseek.html

License

GPL (>= 2)

Recipe

/bioconductor-crisprseek/meta.yaml

Links

biotools: crisprseek

The package includes functions to find potential guide RNAs for the CRISPR editing system including Base Editors and the Prime Editor for input target sequences, optionally filter guide RNAs without restriction enzyme cut site, or without paired guide RNAs, genome-wide search for off-targets, score, rank, fetch flank sequence and indicate whether the target and off-targets are located in exon region or not. Potential guide RNAs are annotated with total score of the top5 and topN off-targets, detailed topN mismatch sites, restriction enzyme cut sites, and paired guide RNAs. The package also output indels and their frequencies for Cas9 targeted sites.

package bioconductor-crisprseek

Versions

1.32.0-0,  1.30.1-0,  1.30.0-0,  1.28.0-0,  1.26.0-0,  1.24.0-1,  1.22.0-0,  1.20.0-0,  1.18.0-0

Depends

• bioconductor-biocgenerics >=0.38.0,<0.39.0

• bioconductor-biocparallel >=1.26.0,<1.27.0

• bioconductor-biostrings >=2.60.0,<2.61.0

• bioconductor-bsgenome >=1.60.0,<1.61.0

• bioconductor-iranges >=2.26.0,<2.27.0

• bioconductor-rhdf5 >=2.36.0,<2.37.0

• bioconductor-s4vectors >=0.30.0,<0.31.0

• r-base >=4.1,<4.2.0a0

• r-data.table

• r-hash

• r-reticulate

• r-seqinr

Required By

• bioconductor-multicrispr

• bioconductor-crisprseekplus

• bioconductor-guideseq

Installation

With an activated Bioconda channel (see 2. Set up channels), install with:

conda install bioconductor-crisprseek

and update with:

conda update bioconductor-crisprseek

or use the docker container:

docker pull quay.io/biocontainers/bioconductor-crisprseek:<tag>

(see bioconductor-crisprseek/tags for valid values for <tag>)

Link to this page

Render an badge with the following MarkDown:

[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat)](http://bioconda.github.io/recipes/bioconductor-crisprseek/README.html)