recipe bioconductor-lintind

Lineage tracing by indels

Homepage:

https://bioconductor.org/packages/3.20/bioc/html/LinTInd.html

License:

MIT + file LICENSE

Recipe:

/bioconductor-lintind/meta.yaml

When we combine gene-editing technology and sequencing technology, we need to reconstruct a lineage tree from alleles generated and calculate the similarity between each pair of groups. FindIndel() and IndelForm() function will help you align each read to reference sequence and generate scar form strings respectively. IndelIdents() function will help you to define a scar form for each cell or read. IndelPlot() function will help you to visualize the distribution of deletion and insertion. TagProcess() function will help you to extract indels for each cell or read. TagDist() function will help you to calculate the similarity between each pair of groups across the indwells they contain. BuildTree() function will help you to reconstruct a tree. PlotTree() function will help you to visualize the tree.

package bioconductor-lintind

(downloads) docker_bioconductor-lintind

Versions:

1.10.0-01.6.0-01.4.0-01.2.0-0

Depends:
  • on bioconductor-biocgenerics >=0.52.0,<0.53.0

  • on bioconductor-biostrings >=2.74.0,<2.75.0

  • on bioconductor-ggtree >=3.14.0,<3.15.0

  • on bioconductor-iranges >=2.40.0,<2.41.0

  • on bioconductor-pwalign >=1.2.0,<1.3.0

  • on bioconductor-s4vectors >=0.44.0,<0.45.0

  • on r-ape

  • on r-base >=4.4,<4.5.0a0

  • on r-cowplot

  • on r-data.tree

  • on r-dplyr

  • on r-ggnewscale

  • on r-ggplot2

  • on r-networkd3

  • on r-pheatmap

  • on r-purrr

  • on r-reshape2

  • on r-rlist

  • on r-stringdist

  • on r-stringr

Additional platforms:

Installation

You need a conda-compatible package manager (currently either pixi, conda, or micromamba) and the Bioconda channel already activated (see Usage). Below, we show how to install with either pixi or conda (for micromamba and mamba, commands are essentially the same as with conda).

Pixi

With pixi installed and the Bioconda channel set up (see Usage), to install globally, run:

pixi global install bioconductor-lintind

to add into an existing workspace instead, run:

pixi add bioconductor-lintind

In the latter case, make sure to first add bioconda and conda-forge to the channels considered by the workspace:

pixi workspace channel add conda-forge
pixi workspace channel add bioconda

Conda

With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run:

conda install bioconductor-lintind

Alternatively, to install into a new environment, run:

conda create -n envname bioconductor-lintind

with envname being the name of the desired environment.

Container

Alternatively, every Bioconda package is available as a container image for usage with your preferred container runtime. For e.g. docker, run:

docker pull quay.io/biocontainers/bioconductor-lintind:<tag>

(see bioconductor-lintind/tags for valid values for <tag>).

Integrated deployment

Finally, note that many scientific workflow management systems directly integrate both conda and container based software deployment. Thus, workflow steps can be often directly annotated to use the package, leading to automatic deployment by the respective workflow management system, thereby improving reproducibility and transparency. Check the documentation of your workflow management system to find out about the integration.

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