recipe bioconductor-lute

Framework for cell size scale factor normalized bulk transcriptomics deconvolution experiments

Homepage:

https://bioconductor.org/packages/3.20/bioc/html/lute.html

License:

Artistic-2.0

Recipe:

/bioconductor-lute/meta.yaml

Provides a framework for adjustment on cell type size when performing bulk transcripomics deconvolution. The main framework function provides a means of reference normalization using cell size scale factors. It allows for marker selection and deconvolution using non-negative least squares (NNLS) by default. The framework is extensible for other marker selection and deconvolution algorithms, and users may reuse the generics, methods, and classes for these when developing new algorithms.

package bioconductor-lute

(downloads) docker_bioconductor-lute

Versions:

1.2.0-0

Depends:
  • on bioconductor-biobase >=2.66.0,<2.67.0

  • on bioconductor-biocgenerics >=0.52.0,<0.53.0

  • on bioconductor-s4vectors >=0.44.0,<0.45.0

  • on bioconductor-scran >=1.34.0,<1.35.0

  • on bioconductor-singlecellexperiment >=1.28.0,<1.29.0

  • on bioconductor-summarizedexperiment >=1.36.0,<1.37.0

  • on r-base >=4.4,<4.5.0a0

  • on r-dplyr

  • on r-ggplot2

Additional platforms:

Installation

You need a conda-compatible package manager (currently either pixi, conda, or micromamba) and the Bioconda channel already activated (see Usage). Below, we show how to install with either pixi or conda (for micromamba and mamba, commands are essentially the same as with conda).

Pixi

With pixi installed and the Bioconda channel set up (see Usage), to install globally, run:

pixi global install bioconductor-lute

to add into an existing workspace instead, run:

pixi add bioconductor-lute

In the latter case, make sure to first add bioconda and conda-forge to the channels considered by the workspace:

pixi workspace channel add conda-forge
pixi workspace channel add bioconda

Conda

With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run:

conda install bioconductor-lute

Alternatively, to install into a new environment, run:

conda create -n envname bioconductor-lute

with envname being the name of the desired environment.

Container

Alternatively, every Bioconda package is available as a container image for usage with your preferred container runtime. For e.g. docker, run:

docker pull quay.io/biocontainers/bioconductor-lute:<tag>

(see bioconductor-lute/tags for valid values for <tag>).

Integrated deployment

Finally, note that many scientific workflow management systems directly integrate both conda and container based software deployment. Thus, workflow steps can be often directly annotated to use the package, leading to automatic deployment by the respective workflow management system, thereby improving reproducibility and transparency. Check the documentation of your workflow management system to find out about the integration.

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