recipe bioconductor-pram

Pooling RNA-seq datasets for assembling transcript models

Homepage:

https://bioconductor.org/packages/3.20/bioc/html/pram.html

License:

GPL (>= 3)

Recipe:

/bioconductor-pram/meta.yaml

Publicly available RNA-seq data is routinely used for retrospective analysis to elucidate new biology. Novel transcript discovery enabled by large collections of RNA-seq datasets has emerged as one of such analysis. To increase the power of transcript discovery from large collections of RNA-seq datasets, we developed a new R package named Pooling RNA-seq and Assembling Models (PRAM), which builds transcript models in intergenic regions from pooled RNA-seq datasets. This package includes functions for defining intergenic regions, extracting and pooling related RNA-seq alignments, predicting, selected, and evaluating transcript models.

package bioconductor-pram

(downloads) docker_bioconductor-pram

Versions:
1.26.0-01.22.0-01.18.0-01.16.0-01.14.0-01.10.0-01.8.0-01.6.0-11.6.0-0

1.26.0-01.22.0-01.18.0-01.16.0-01.14.0-01.10.0-01.8.0-01.6.0-11.6.0-01.4.0-01.2.0-01.0.0-1

Depends:
  • on bioconductor-biocgenerics >=0.56.0,<0.57.0

  • on bioconductor-biocparallel >=1.44.0,<1.45.0

  • on bioconductor-genomicalignments >=1.46.0,<1.47.0

  • on bioconductor-genomicranges >=1.62.0,<1.63.0

  • on bioconductor-iranges >=2.44.0,<2.45.0

  • on bioconductor-rsamtools >=2.26.0,<2.27.0

  • on bioconductor-rtracklayer >=1.70.0,<1.71.0

  • on bioconductor-s4vectors >=0.48.0,<0.49.0

  • on bioconductor-seqinfo >=1.0.0,<1.1.0

  • on r-base >=4.5,<4.6.0a0

  • on r-data.table >=1.11.8

Additional platforms:

Installation

You need a conda-compatible package manager (currently either pixi, conda, or micromamba) and the Bioconda channel already activated (see Usage). Below, we show how to install with either pixi or conda (for micromamba and mamba, commands are essentially the same as with conda).

Pixi

With pixi installed and the Bioconda channel set up (see Usage), to install globally, run:

pixi global install bioconductor-pram

to add into an existing workspace instead, run:

pixi add bioconductor-pram

In the latter case, make sure to first add bioconda and conda-forge to the channels considered by the workspace:

pixi workspace channel add conda-forge
pixi workspace channel add bioconda

Conda

With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run:

conda install bioconductor-pram

Alternatively, to install into a new environment, run:

conda create -n envname bioconductor-pram

with envname being the name of the desired environment.

Container

Alternatively, every Bioconda package is available as a container image for usage with your preferred container runtime. For e.g. docker, run:

docker pull quay.io/biocontainers/bioconductor-pram:<tag>

(see bioconductor-pram/tags for valid values for <tag>).

Integrated deployment

Finally, note that many scientific workflow management systems directly integrate both conda and container based software deployment. Thus, workflow steps can be often directly annotated to use the package, leading to automatic deployment by the respective workflow management system, thereby improving reproducibility and transparency. Check the documentation of your workflow management system to find out about the integration.

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