recipe craq

Pinpoint assembly errors for genomic assessing and correcting.

Homepage:

https://github.com/JiaoLaboratory/CRAQ

License:

MIT / MIT

Recipe:

/craq/meta.yaml

CRAQ (Clipping Reveals Assembly Quality) is a reference-free genome assembly evaluator that can assess the accuracy of assembled genomic sequences and provide detailed assembly quality assessment from multiple perspectives. It can report precise locations of small-scale Clip-based Regional Errors (CREs), large-scale Clip-based Structural Errors (CSEs), as well as regional and overall AQI metrics (R-AQI & S-AQI) for assembly evaluation. Through evaluating a large set of genome assemblies with different qualities, we classified genomes as following: AQI > 90, reference quality; AQI from 80-90, high quality; AQI from 60-80, draft quality; and AQI < 60, low quality. CRAQ also considered haplotype features which is important for identifying true misassembly. It can output coordinates of regional heterozygous variants (CRHs) and coordinates of structural heterozygous variants (CSHs) based on the ratio of clipped alignments and mapping coverage. Moreover, CRAQ detects potential chimeric contigs and break them at conflict breakpoints for assembly correction. This document has the information on how to run CRAQ.

package craq

(downloads) docker_craq

versions:

1.0.9-0

depends minimap2:

>=2.17

depends perl:

>=5

depends python:

>=3.7

depends python_circos:

requirements:

Installation

You need a conda-compatible package manager (currently either micromamba, mamba, or conda) and the Bioconda channel already activated (see set-up-channels).

While any of above package managers is fine, it is currently recommended to use either micromamba or mamba (see here for installation instructions). We will show all commands using mamba below, but the arguments are the same for the two others.

Given that you already have a conda environment in which you want to have this package, install with:

   mamba install craq

and update with::

   mamba update craq

To create a new environment, run:

mamba create --name myenvname craq

with myenvname being a reasonable name for the environment (see e.g. the mamba docs for details and further options).

Alternatively, use the docker container:

   docker pull quay.io/biocontainers/craq:<tag>

(see `craq/tags`_ for valid values for ``<tag>``)

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