recipe fba

Tools for single-cell feature barcoding analysis. Citation: Duan, et al (2021) <doi:10.1093/bioinformatics/btab375>.

Homepage:

https://github.com/jlduan/fba

Documentation:

https://jlduan.github.io/fba

License:

MIT

Recipe:

/fba/meta.yaml

'fba is a flexible and streamlined toolbox for quality control, quantification, demultiplexing of various single-cell feature barcoding assays. It can be applied to customized feature barcoding specifications, including different CRISPR constructs or targeted enriched transcripts. fba allows users to customize a wide range of parameters for the quantification and demultiplexing process. fba also has a user-friendly quality control module, which is helpful in troubleshooting feature barcoding experiments.'

package fba

(downloads) docker_fba

Versions:

0.0.13-00.0.12-00.0.11-00.0.10.post1-0

Depends:
  • on dnaio >=0.10.0

  • on hdbscan >=0.8.21

  • on matplotlib-base >=3.3

  • on numpy >=1.19.0

  • on pandas >=1.0.0

  • on polyleven >=0.5

  • on pyclustering >=0.10.1

  • on pysam >=0.14.0

  • on python >=3.6

  • on regex

  • on scikit-learn >=0.23.0

  • on scipy >=1.5.0

  • on seaborn >=0.10.0

  • on statsmodels >=0.11.1

  • on umap-learn

  • on umi_tools >=1.0.0

Additional platforms:

Installation

You need a conda-compatible package manager (currently either pixi, conda, or micromamba) and the Bioconda channel already activated (see Usage). Below, we show how to install with either pixi or conda (for micromamba and mamba, commands are essentially the same as with conda).

Pixi

With pixi installed and the Bioconda channel set up (see Usage), to install globally, run:

pixi global install fba

to add into an existing workspace instead, run:

pixi add fba

In the latter case, make sure to first add bioconda and conda-forge to the channels considered by the workspace:

pixi workspace channel add conda-forge
pixi workspace channel add bioconda

Conda

With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run:

conda install fba

Alternatively, to install into a new environment, run:

conda create -n envname fba

with envname being the name of the desired environment.

Container

Alternatively, every Bioconda package is available as a container image for usage with your preferred container runtime. For e.g. docker, run:

docker pull quay.io/biocontainers/fba:<tag>

(see fba/tags for valid values for <tag>).

Integrated deployment

Finally, note that many scientific workflow management systems directly integrate both conda and container based software deployment. Thus, workflow steps can be often directly annotated to use the package, leading to automatic deployment by the respective workflow management system, thereby improving reproducibility and transparency. Check the documentation of your workflow management system to find out about the integration.

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