recipe bioconductor-ddct

The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.

Homepage

https://bioconductor.org/packages/3.9/bioc/html/ddCt.html

License

LGPL-3

Recipe

/bioconductor-ddct/meta.yaml

Links

biotools: ddct, doi: 10.1038/nmeth.3252

package bioconductor-ddct

(downloads) docker_bioconductor-ddct

Versions

1.38.0-0, 1.36.0-0, 1.34.0-0

Depends
Required By

Installation

With an activated Bioconda channel (see 2. Set up channels), install with:

conda install bioconductor-ddct

and update with:

conda update bioconductor-ddct

or use the docker container:

docker pull quay.io/biocontainers/bioconductor-ddct:<tag>

(see bioconductor-ddct/tags for valid values for <tag>)