recipe bioconductor-htqpcr

Automated analysis of high-throughput qPCR data

Homepage:

https://bioconductor.org/packages/3.18/bioc/html/HTqPCR.html

License:

Artistic-2.0

Recipe:

/bioconductor-htqpcr/meta.yaml

Links:

biotools: htqpcr

Analysis of Ct values from high throughput quantitative real-time PCR (qPCR) assays across multiple conditions or replicates. The input data can be from spatially-defined formats such ABI TaqMan Low Density Arrays or OpenArray; LightCycler from Roche Applied Science; the CFX plates from Bio-Rad Laboratories; conventional 96- or 384-well plates; or microfluidic devices such as the Dynamic Arrays from Fluidigm Corporation. HTqPCR handles data loading, quality assessment, normalization, visualization and parametric or non-parametric testing for statistical significance in Ct values between features (e.g. genes, microRNAs).

package bioconductor-htqpcr

(downloads) docker_bioconductor-htqpcr

Versions:
1.64.0-0,  1.56.0-0,  1.54.0-0,  1.52.0-0,  1.48.0-0,  1.46.0-0,  1.44.0-1,  1.44.0-0,  1.42.0-0, 

1.64.0-0,  1.56.0-0,  1.54.0-0,  1.52.0-0,  1.48.0-0,  1.46.0-0,  1.44.0-1,  1.44.0-0,  1.42.0-0,  1.40.0-0,  1.38.0-1,  1.36.0-0,  1.34.0-0,  1.32.0-0

Depends:

  • on bioconductor-affy >=1.88.0,<1.89.0

  • on bioconductor-biobase >=2.70.0,<2.71.0

  • on bioconductor-limma >=3.66.0,<3.67.0

  • on r-base >=4.5,<4.6.0a0

  • on r-gplots

  • on r-rcolorbrewer

Additional platforms:

Installation

You need a conda-compatible package manager (currently either pixi, conda, or micromamba) and the Bioconda channel already activated (see Usage). Below, we show how to install with either pixi or conda (for micromamba and mamba, commands are essentially the same as with conda).

Pixi

With pixi installed and the Bioconda channel set up (see Usage), to install globally, run:

pixi global install bioconductor-htqpcr

to add into an existing workspace instead, run:

pixi add bioconductor-htqpcr

In the latter case, make sure to first add bioconda and conda-forge to the channels considered by the workspace:

pixi workspace channel add conda-forge
pixi workspace channel add bioconda

Conda

With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run:

conda install bioconductor-htqpcr

Alternatively, to install into a new environment, run:

conda create -n envname bioconductor-htqpcr

with envname being the name of the desired environment.

Container

Alternatively, every Bioconda package is available as a container image for usage with your preferred container runtime. For e.g. docker, run:

docker pull quay.io/biocontainers/bioconductor-htqpcr:<tag>

(see bioconductor-htqpcr/tags for valid values for <tag>).

Integrated deployment

Finally, note that many scientific workflow management systems directly integrate both conda and container based software deployment. Thus, workflow steps can be often directly annotated to use the package, leading to automatic deployment by the respective workflow management system, thereby improving reproducibility and transparency. Check the documentation of your workflow management system to find out about the integration.

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